From 1835 through 1957, Worthington's chief law enforcement official was known as the "marshal." In December 1957, the title "Chief of Police" was created by city ordinance. Chief Edward J. Lewis was serving as marshal at the time, and became Worthington's first official police chief. 

Abner P. Pinney, 1836-1837
James Y. Beers, 1845-1846
Charles Martin, 1847-1848
James Y. Beers, 1851-1853
John M. Gilbert, 1856-1857
James Jaycox, 1858-1859
Joseph B. Tozer, 1859-1860
Charles Martin, 1860-1861
James Y. Beers, 1861-1862
John M. Gilbert, 1863-1865
Joseph B. Tozer, 1867-1868
Walter Foss, 1870-1871
Andrew Powell, 1872-1873
James Barker, 1874-1876
James Y. Beers, 1876-1878
Samuel M. Sullivan, 1878-1879
James Y. Beers, 1879-1880
William E. White, 1880-1882
Mort S. Hiser, 1882-1883
James M. Morgan, 1883-1884
Henry B. Stickney, 1884-1885
Mort S. Hiser, 1886-1888
John A. Leckliter, 1889-1891
Charles Kiner, 1891-1892
John D. McDonald, 1901-1901
Leroy F. Bosley, 1901-1902
Charles E. Wilson, 1905-1907
Charles P. Michael, 1907-1909
Thomas E. Albert, 1909-1917
Charles E. Wilson, 1917-1920
James H. Taladay, 1924-1954
Edward J. Lewis, 1954-1963
Paul R. Abbott, 1964-1992
Wayne I. McCoy, 1992-2002
Michael E. Mauger, 2002-2011
James Mosic, 2011-2016
Jerry Strait, 2016-2019
Robert K. Ware, II, 2019-2022
Eric Grile, 2023-2024
Stephen Mylett, 2024-